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hybez hybridisation system  (Advanced Cell Diagnostics Inc)

 
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    Advanced Cell Diagnostics Inc hybez hybridisation system
    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ <t>hybridisation-immunohistochemistry</t> (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Hybez Hybridisation System, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybez hybridisation system/product/Advanced Cell Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    hybez hybridisation system - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "P2X 7 R influences tau aggregate burden in human tauopathies and shows distinct signalling in microglia and astrocytes"

    Article Title: P2X 7 R influences tau aggregate burden in human tauopathies and shows distinct signalling in microglia and astrocytes

    Journal: Brain, Behavior, and Immunity

    doi: 10.1016/j.bbi.2023.09.011

    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ hybridisation-immunohistochemistry (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ hybridisation-immunohistochemistry (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: In Situ, Hybridization, Immunohistochemistry, Control, RNAscope, Staining, Expressing, Transgenic Assay



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    hybez hybridisation system  (Advanced Cell Diagnostics Inc)
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    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ <t>hybridisation-immunohistochemistry</t> (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ <t>hybridisation-immunohistochemistry</t> (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    hybridisation oven hybez hybridization system (220vac) with acd ezbatch slide system  (Advanced Cell Diagnostics Inc)
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    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ <t>hybridisation-immunohistochemistry</t> (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ hybridisation-immunohistochemistry (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Brain, Behavior, and Immunity

    Article Title: P2X 7 R influences tau aggregate burden in human tauopathies and shows distinct signalling in microglia and astrocytes

    doi: 10.1016/j.bbi.2023.09.011

    Figure Lengend Snippet: P2RX7 mRNA localises to GFAP + astrocytes and CD68 + microglia surrounding Aβ plaques in AD brain. a) Representative in-situ hybridisation-immunohistochemistry (ISH-IHC) images of brain sections from frontal cortex (BA9) of human control brain (Braak stage 0-II) hybridised with RNAscope probes against P2RX7 mRNA (red puncta, indicated by arrowheads) and immunolabelled with antibodies against GFAP and βIII-tubulin (blue/grey chromogenic stain) and CD68 (diaminobenzidine [DAB] stain). n = 3. Representative images of P2RX7 RNAscope performed in sections from three Braak stage III-IV and three Braak stage V-VI AD cases immunolabelled with antibodies against (b) GFAP , (c) CD68 and (d) Aβ (6E10). Arrowheads indicate representative P2RX7 mRNA puncta in GFAP or CD68 immunopositive cells, or in the vicinity of Aβ deposits. Insets indicate regions of each image displayed at a higher magnification. n = 3. Scale bar: 10 µm. e) Volcano plots showing changes in expression of P2r transcripts in transgenic CRND8 mouse brains versus non-transgenic controls at 3, 6, 12 and 20 months obtained from the AMP-AD Knowledge Portal ( https://doi.org/10.7303/syn3157182) . Dotted lines indicate the cut-off p-value of 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: In brief, slides were baked in a HybEz hybridisation system (ACD Bio) or a hybridiser HB-1D oven (Akribis Scientific Limited) at 60 °C for 30 min. FFPE-sections were deparaffinised in xylene and 100 % ethanol.

    Techniques: In Situ, Hybridization, Immunohistochemistry, Control, RNAscope, Staining, Expressing, Transgenic Assay